Skip to main content
Addgene

pGGF_RT_FAST_Venus
(Plasmid #136980)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136980 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    seed coat marker, replaced RFP w/ Venus. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
  • Species
    Synthetic
  • Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G > A, 1427 A>G, 1454 C > T, 1457 A > G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate resistance cassette module, containing the seed coat marker --> DOI:10.1111/j.1365-313X.2009.04060.x

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF_RT_FAST_Venus was a gift from Klaus Theres (Addgene plasmid # 136980 ; http://n2t.net/addgene:136980 ; RRID:Addgene_136980)
Related items:
Commonly requested with: