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Addgene

pGGF_RT_FAST_RFP
(Plasmid #136979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136979 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    seed coat marker RFP
  • Species
    Synthetic
  • Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G > A, 1427 A>G, 1454 C > T, 1457 A > G

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGGF_RT_FAST_RFP was a gift from Klaus Theres (Addgene plasmid # 136979 ; http://n2t.net/addgene:136979 ; RRID:Addgene_136979)

  • For your References section:

    Mutations in the miR396 binding site of the growth-regulating factor gene VvGRF4 modulate inflorescence architecture in grapevine. Rossmann S, Richter R, Sun H, Schneeberger K, Topfer R, Zyprian E, Theres K. Plant J. 2019 Oct 30. doi: 10.1111/tpj.14588. 10.1111/tpj.14588 PubMed 31663642
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