pGGD_RT_2NLS
(Plasmid
#136977)
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PurposeGoldengate/Greengate module nuclear localization signal
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSV40 NLS
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For use in the Greengate system (DOI: 10.1371/journal.pone.0083043, Addgene: Kit # 1000000036) or similar Goldengate-based systems. This plasmid carries a Greengate C-tag module, containing 2 NLS's in sequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGGD_RT_2NLS was a gift from Klaus Theres (Addgene plasmid # 136977 ; http://n2t.net/addgene:136977 ; RRID:Addgene_136977)