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PurposeReporter vector encoding firefly luciferase and TurboRFP fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNL4-3
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFirefly luciferase and TurboRFP
- Promoter CMV
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Tag
/ Fusion Protein
- V5 tag
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 rev
- 3′ sequencing primer M13 fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byLuc2 coding region: pGL4.20[luc2/Puro] vector (Promega) TurboRFP coding region: pTurboRFP-N vector (Evrogen)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL(CMV)Luc2-Turbo RFP/CMV/WPREdU3 was a gift from Jakob Reiser (Addgene plasmid # 136959 ; http://n2t.net/addgene:136959 ; RRID:Addgene_136959) -
For your References section:
A patient-derived orthotopic xenograft model enabling human high-grade urothelial cell carcinoma of the bladder tumor implantation, growth, angiogenesis, and metastasis. Gills J, Moret R, Zhang X, Nelson J, Maresh G, Hellmers L, Canter D, Hudson M, Halat S, Matrana M, Marino MP, Reiser J, Shuh M, Laborde E, Latsis M, Talwar S, Bardot S, Li L. Oncotarget. 2018 Aug 24;9(66):32718-32729. doi: 10.18632/oncotarget.26024. eCollection 2018 Aug 24. 10.18632/oncotarget.26024 PubMed 30220977