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Addgene

pDiySpike3
(Plasmid #136950)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136950 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    Joachim Messing
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DiySpike3
  • Species
    Synthetic
  • Insert Size (bp)
    1084
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDiySpike3 was a gift from Rickard Sandberg (Addgene plasmid # 136950 ; http://n2t.net/addgene:136950 ; RRID:Addgene_136950)

  • For your References section:

    Single-cell RNA counting at allele and isoform resolution using Smart-seq3. Hagemann-Jensen M, Ziegenhain C, Chen P, Ramskold D, Hendriks GJ, Larsson AJM, Faridani OR, Sandberg R. Nat Biotechnol. 2020 Jun;38(6):708-714. doi: 10.1038/s41587-020-0497-0. Epub 2020 May 4. 10.1038/s41587-020-0497-0 PubMed 32518404
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