Skip to main content
Addgene

pJBL7004
(Plasmid #136943)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2854
  • Total vector size (bp) 2967
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    malachite green aptamer (MGA)
  • Species
    Synthetic
  • Insert Size (bp)
    123
  • Promoter pT7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTTTCCCAGTCACGAC
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJBL7004 was a gift from Julius Lucks (Addgene plasmid # 136943 ; http://n2t.net/addgene:136943 ; RRID:Addgene_136943)

  • For your References section:

    Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry. Silverman AD, Kelley-Loughnane N, Lucks JB, Jewett MC. ACS Synth Biol. 2019 Feb 15;8(2):403-414. doi: 10.1021/acssynbio.8b00430. Epub 2019 Jan 29. 10.1021/acssynbio.8b00430 PubMed 30596483
Related items:
Commonly requested with: