cmv 16 E7 L67K
(Plasmid
#13694)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV bam neo
- Backbone size w/o insert (bp) 6550
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 16 E7 L67K
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Alt nameHPV 16 E7
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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Mutationaa 67 from L to K
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GenBank IDAY089955
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Entrez GeneE7 (a.k.a. HpV16gp2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (destroyed during cloning)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer B glob intron-F
- 3′ sequencing primer B glob-pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cmv 16 E7 L67K was a gift from Karl Munger (Addgene plasmid # 13694 ; http://n2t.net/addgene:13694 ; RRID:Addgene_13694)