AAVS1-Puro cTnT FUCCI
(Plasmid #136935)

• Purpose: donor plasmid for cardiac-specific FUCCI expression in human cells
• Depositing Lab: Xiaoping Bao
• Publication: Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352.

Backbone

• Vector backbone: AAVS1-Puro
• Backbone size w/o insert (bp): 11266
• Total vector size (bp): 11878
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Troponin T promoter
• Alt name: cTnT
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 612
• Promoter: cTnT

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cTnT promoter was cloned from Addgene plasmid # 46027, a gift from Dr. John Gearhart Lab.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry