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Addgene

cmv 16 E7 E46A
(Plasmid #13693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV bam neo
  • Backbone size w/o insert (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 16 E7 E46A
  • Alt name
    HPV 16 E7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    aa 46 changed from E to A
  • GenBank ID
    AY089955
  • Entrez Gene
    E7 (a.k.a. HpV16gp2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (destroyed during cloning)
  • 5′ sequencing primer B glob intron-F
  • 3′ sequencing primer B glob-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cmv 16 E7 E46A was a gift from Karl Munger (Addgene plasmid # 13693 ; http://n2t.net/addgene:13693 ; RRID:Addgene_13693)
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