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PurposeExpresses ABEmax-NRCH in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322 CMV
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameABEmax-NRCH
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SpeciesSynthetic; APOBEC1 is from rat (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized)
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Mutationsee manuscript
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert has leaky expression during bacterial propagation and can result in a higher than expected spontaneous mutation rate. The insert should always be sequence validated before use.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-ABEmax-NRCH was a gift from David Liu (Addgene plasmid # 136923 ; http://n2t.net/addgene:136923 ; RRID:Addgene_136923) -
For your References section:
Continuous evolution of SpCas9 variants compatible with non-G PAMs. Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek Z, Newby GA, Rees HA, Liu DR. Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 10.1038/s41587-020-0412-8. Epub 2020 Feb 10. 10.1038/s41587-020-0412-8 PubMed 32042170