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Addgene

BPK880-pCAG-DmrC-NLS-3xFLAG-VP64
(Plasmid #136912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-GFP (Addgene # 11179)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pCAG-DmrC-NLS-3xFLAG-VP64
  • Species
    H. sapiens (human)
  • Promoter Chicken Beta Actin

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer oBK23-R(5'-CAGAGGGAAAAAGATCTCAGTGG-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK880-pCAG-DmrC-NLS-3xFLAG-VP64 was a gift from Keith Joung (Addgene plasmid # 136912 ; http://n2t.net/addgene:136912 ; RRID:Addgene_136912)

  • For your References section:

    Augmenting and directing long-range CRISPR-mediated activation in human cells. Tak YE, Horng JE, Perry NT, Schultz HT, Iyer S, Yao Q, Zou LS, Aryee MJ, Pinello L, Joung JK. Nat Methods. 2021 Sep;18(9):1075-1081. doi: 10.1038/s41592-021-01224-1. Epub 2021 Aug 5. 10.1038/s41592-021-01224-1 PubMed 34354266
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