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Addgene

pLenti-FNLSHiFi-P2A-Puro
(Plasmid #136902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Adapted from pLentiCRISPRv1
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FNLSHiFi
  • Species
    Synthetic
  • Mutation
    R691A
  • Promoter EF1s
  • Tags / Fusion Proteins
    • FLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-FNLSHiFi-P2A-Puro was a gift from Lukas Dow (Addgene plasmid # 136902 ; http://n2t.net/addgene:136902 ; RRID:Addgene_136902)

  • For your References section:

    GO: a functional reporter system to identify and enrich base editing activity. Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. 10.1093/nar/gkaa124 PubMed 32112097
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