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Addgene

pRRL-GFPGO2(NGT)-PGK-Neo
(Plasmid #136892)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136892 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFPGO2(NGT)
  • Species
    Synthetic
  • Mutation
    ATG>ACG
  • Promoter SFFV
  • Tag / Fusion Protein
    • 2xNLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRL-GFPGO2(NGT)-PGK-Neo was a gift from Lukas Dow (Addgene plasmid # 136892 ; http://n2t.net/addgene:136892 ; RRID:Addgene_136892)

  • For your References section:

    GO: a functional reporter system to identify and enrich base editing activity. Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE. Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. 10.1093/nar/gkaa124 PubMed 32112097
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