TetO-FUW-FLAG-NLS-EnR-MCS_N-term
(Plasmid
#136884)
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PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTetO-FUW
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameengrailed repressor domain
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SpeciesD. melanogaster (fly)
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Entrez Geneen (a.k.a. Dmel_CG9015, 153867_at, Apa, CG9015, Dmel\CG9015, EN, En, EnR, Eng, Engr, Engrailed, Engrailed/Invected, Es, V, en1, spa2, spt)
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Tag
/ Fusion Protein
- Flag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TetO-FUW-FLAG-NLS-EnR-MCS_N-term was a gift from Moritz Mall (Addgene plasmid # 136884 ; http://n2t.net/addgene:136884 ; RRID:Addgene_136884) -
For your References section:
Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions. Adrian-Segarra JM, Weigel B, Mall M. Methods Mol Biol. 2021;2352:227-236. doi: 10.1007/978-1-0716-1601-7_15. 10.1007/978-1-0716-1601-7_15 PubMed 34324190