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Addgene

cmv 16 E7 del PTLHE
(Plasmid #13688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV bam neo
  • Backbone size w/o insert (bp) 6550
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPV 16 E7 del PTLHE
  • Alt name
    HPV 16 E7 del 6-10
  • Alt name
    HPV 16 E7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    del aa 6-10
  • GenBank ID
    AY089955
  • Entrez Gene
    E7 (a.k.a. HpV16gp2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer B glob intron-F
  • 3′ sequencing primer B glob-pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cmv 16 E7 del PTLHE was a gift from Karl Munger (Addgene plasmid # 13688 ; http://n2t.net/addgene:13688 ; RRID:Addgene_13688)

  • For your References section:

    Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7. Gonzalez SL, Stremlau M, He X, Basile JR, Munger K. J Virol. 2001 Aug . 75(16):7583-91. 10.1128/JVI.75.16.7583-7591.2001 PubMed 11462030
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