pEBTetBl-CLIP-Cep78
(Plasmid
#136876)
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PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEBTetBl
- Backbone size w/o insert (bp) 10957
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCLIP-Cep78
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)3057
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Entrez GeneCEP78 (a.k.a. IP63, C9orf81, FLJ12643, FLJ52093, MGC135040)
- Promoter CMV-TetO2
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Tag
/ Fusion Protein
- FLAG-tag / His-tag (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBTetBl-CLIP-Cep78 was a gift from Kai Johnsson (Addgene plasmid # 136876 ; http://n2t.net/addgene:136876 ; RRID:Addgene_136876) -
For your References section:
Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex. Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin N, Gonczy P, Johnsson K. Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17. 10.1016/j.cub.2012.12.030 PubMed 23333316
