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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13686 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV bam neo
- Backbone size w/o insert (bp) 6550
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV 16 E7
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SpeciesH. sapiens (human)
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Insert Size (bp)300
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GenBank IDAY089955
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Entrez GeneE7 (a.k.a. HpV16gp2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer B glob intron-F
- 3′ sequencing primer B glob-pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cmv 16 E7 was a gift from Karl Munger (Addgene plasmid # 13686 ; http://n2t.net/addgene:13686 ; RRID:Addgene_13686) -
For your References section:
Degradation of the retinoblastoma tumor suppressor by the human papillomavirus type 16 E7 oncoprotein is important for functional inactivation and is separable from proteasomal degradation of E7. Gonzalez SL, Stremlau M, He X, Basile JR, Munger K. J Virol. 2001 Aug . 75(16):7583-91. 10.1128/JVI.75.16.7583-7591.2001 PubMed 11462030