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Addgene

pEBTetBl-CLIP-Sfi1
(Plasmid #136842)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136842 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBTetBl
  • Backbone size w/o insert (bp) 10957
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLIP-hSfi1
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    4617
  • Entrez Gene
    SFI1 (a.k.a. PISD, PPP1R139, hSfi1p)
  • Promoter CMV-TetO2
  • Tag / Fusion Protein
    • FLAG-tag / His-tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBTetBl-CLIP-Sfi1 was a gift from Kai Johnsson (Addgene plasmid # 136842 ; http://n2t.net/addgene:136842 ; RRID:Addgene_136842)

  • For your References section:

    Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex. Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin N, Gonczy P, Johnsson K. Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17. 10.1016/j.cub.2012.12.030 PubMed 23333316
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