pEBTetDBl-CLIP-α-tubulin
(Plasmid #136841)

• Purpose: Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag
• Depositing Lab: Kai Johnsson
• Publication: Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17.

Backbone

• Vector backbone: pEBTetDBl
• Backbone size w/o insert (bp): 10641
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CLIP-TUBA
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2229
• Entrez Gene: DNMBP (a.k.a. ARHGEF36, CTRCT48, TUBA)
• Promoter: CMV-TetO2
• Tag / Fusion Protein:
  • FLAG-tag / His-tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PolyAdenylation RNA deletion - low protein expression level