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pEBTetD-SNAP-Cep290
(Plasmid #136805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBTetD
  • Backbone size w/o insert (bp) 10958
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SNAP-Cep290
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1368
  • Entrez Gene
    CEP290 (a.k.a. 3H11Ag, BBS14, CT87, JBTS5, LCA10, MKS4, NPHP6, POC3, SLSN6, rd16)
  • Promoter CMV-TetO2
  • Tag / Fusion Protein
    • FLAG-tag / His-tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PolyAdenylation RNA deletion - low protein expression level

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBTetD-SNAP-Cep290 was a gift from Kai Johnsson (Addgene plasmid # 136805 ; http://n2t.net/addgene:136805 ; RRID:Addgene_136805)

  • For your References section:

    Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex. Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin N, Gonczy P, Johnsson K. Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17. 10.1016/j.cub.2012.12.030 PubMed 23333316
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