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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep1318
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Alt nameBeta actin promoter
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Insert Size (bp)7500
- Promoter Beta Actin
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal 1 (not destroyed)
- 3′ cloning site Aat II (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB-actin was a gift from Karl Munger (Addgene plasmid # 13680 ; http://n2t.net/addgene:13680 ; RRID:Addgene_13680) -
For your References section:
The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Münger K, Phelps WC, Bubb V, Howley PM, Schlegel R. J Virol. 1989 Oct . 63(10):4417-21. PubMed 2476573