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Addgene

pB-actin
(Plasmid #13680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p1318
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Alt name
    Beta actin promoter
  • Insert Size (bp)
    7500
  • Promoter Beta Actin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal 1 (not destroyed)
  • 3′ cloning site Aat II (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-actin was a gift from Karl Munger (Addgene plasmid # 13680 ; http://n2t.net/addgene:13680 ; RRID:Addgene_13680)

  • For your References section:

    The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Münger K, Phelps WC, Bubb V, Howley PM, Schlegel R. J Virol. 1989 Oct . 63(10):4417-21. PubMed 2476573
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