pRK-Myc-Smurf2 (CG)
(Plasmid #13679)

• Depositing Lab: Ying Zhang
• Publication: Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):974-9.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Smurf2 (C716G)
• Alt name: Smurf2 C716G
• Species: H. sapiens (human)
• Insert Size (bp): 2241
• Mutation: Smurf2 with a C416 to G substution.
• GenBank ID: NM_022739
• Entrez Gene: SMURF2
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry