pEBTetD-SNAP-Centrin2
(Plasmid #136785)

• Purpose: Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag
• Depositing Lab: Kai Johnsson
• Publication: Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. Epub 2013 Jan 17.

Backbone

• Vector backbone: pEBTetD
• Backbone size w/o insert (bp): 10960
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNAP-Centrin2
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1392
• Entrez Gene: CETN2 (a.k.a. CALT, CEN2)
• Promoter: CMV-TetO2
• Tag / Fusion Protein:
  • FLAG-tag / His-tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PolyAdenylation RNA deletion - low protein expression level