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Addgene

pRK-Myc-Smurf2
(Plasmid #13678)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smurf2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2241
  • GenBank ID
    NM_022739
  • Entrez Gene
    SMURF2
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK-Myc-Smurf2 was a gift from Ying Zhang (Addgene plasmid # 13678 ; http://n2t.net/addgene:13678 ; RRID:Addgene_13678)

  • For your References section:

    Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase. Zhang Y, Chang C, Gehling DJ, Hemmati-Brivanlou A, Derynck R. Proc Natl Acad Sci U S A. 2001 Jan 30. 98(3):974-9. 10.1073/pnas.98.3.974 PubMed 11158580
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