pISH-Olfr1395
(Plasmid
#136731)
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PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T-Easy
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Vector typein situ probe
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOlfr1395
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SpeciesM. musculus (mouse)
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MutationISH Probe insert, D175G and L220F- please dep. comment below
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ISH probe for mouse gene: Olfr1395 aka MOR277-1
Depositor confirms D175G and L220F mutations do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Olfr1395 was a gift from Peter Mombaerts (Addgene plasmid # 136731 ; http://n2t.net/addgene:136731 ; RRID:Addgene_136731) -
For your References section:
The Zonal Organization of Odorant Receptor Gene Choice in the Main Olfactory Epithelium of the Mouse. Zapiec B, Mombaerts P. Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 10.1016/j.celrep.2020.02.110. 10.1016/j.celrep.2020.02.110 PubMed 32209480