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Addgene

pTet-ctl
(Plasmid #136693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tet-pLKO-puro (Plasmid #21915)
  • Backbone size (bp) 8762
  • Modifications to backbone
    Tet-pLKO-puro (Plasmid #21915) was digested with AgeI and EcoRI and filled-in 5’ overhangs with Klenow DNA polymerase. The resulting pTet-ctl plasmid was confirmed by single digestion with Nco (Expected bands: 3022bp, 5740bp) or XhoI (Expected bands: 8447bp, 190bp, 125bp).
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTet-ctl was a gift from LuZhe Sun (Addgene plasmid # 136693 ; http://n2t.net/addgene:136693 ; RRID:Addgene_136693)

  • For your References section:

    Differential effects of GLI2 and GLI3 in regulating cervical cancer malignancy in vitro and in vivo. Zhu H, Xia L, Shen Q, Zhao M, Gu X, Bouamar H, Wang B, Sun LZ, Zhu X. Lab Invest. 2018 Nov;98(11):1384-1396. doi: 10.1038/s41374-018-0089-5. Epub 2018 Jul 2. 10.1038/s41374-018-0089-5 PubMed 29967343
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