pTet-GLI2shR
(Plasmid #136691)

• Purpose: Inducible Gli2 shRNA lentiviral plasmid (target sequence: human GLI2 5′CCGGCCTGGCATGACTACCACTATGCTCGAGCATAGTGGTAGTCATGCCAGGTTTTTG 3′)
• Depositing Lab: LuZhe Sun
• Publication: Zhu et al Lab Invest. 2018 Nov;98(11):1384-1396. doi: 10.1038/s41374-018-0089-5. Epub 2018 Jul 2.

Backbone

• Vector backbone: Tet-pLKO-puro (Plasmid #21915)
• Backbone size w/o insert (bp): 8758
• Total vector size (bp): 8815
• Modifications to backbone: Tet-pLKO-puro (Plasmid #21915) was digested with AgeI and EcoRI to insert Gli2 shRNA-coding sequence (aattaaaaacctggcatgactaccactatgctcgagcatagtggtagtcatgccagg). The resulting pTet-GLI2shR plasmid was confirmed by single digestion with XhoI (Expected bands: 8447bp, 190bp, 136bp, 42bp)
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: shRNA_humanGli2
• gRNA/shRNA sequence: aattaaaaacctggcatgactaccactatgctcgagcatagtggtagtcatgccagg
• Species: H. sapiens (human)
• GenBank ID: NM_001371271
• Entrez Gene: GLI2 (a.k.a. CJS, HPE9, PHS2, THP1, THP2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGCAGGGATATTCACCATTATCGTTTCAGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry