BPV00605 pET21GG2-His(6)-BECN(1-265)
(Plasmid #136686)

• Purpose: Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag
• Depositing Lab: Colin Garvie
• Publication: Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 10.1021/acs.biochem.7b00936. Epub 2017 Dec 14.

Backbone

• Vector backbone: pET21b
• Total vector size (bp): 6339
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Beclin-1 IDR + CC
• Alt name: Intrinsically disordered region, BH3 motif, and Coiled-coil domain
• Species: H. sapiens (human)
• Insert Size (bp): 867
• Mutation: DNA optimized for E. coli expression
• Entrez Gene: BECN1 (a.k.a. ATG6, VPS30, beclin1)
• Promoter: T7
• Tag / Fusion Protein:
  • Hexa-Histadine (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry