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Addgene

pVAX_CBI_AcrIIA6_DT1
(Plasmid #136668)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136668 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pVAX_CBI
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrIIA6_DT1
  • Species
    H. sapiens (human), Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • No tag, No NLS

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVAX_CBI_AcrIIA6_DT1 was a gift from Yannick Doyon (Addgene plasmid # 136668 ; http://n2t.net/addgene:136668 ; RRID:Addgene_136668)
  • For your References section:

    Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. 10.1038/s41467-018-05092-w PubMed 30046034