pVAX_CBI_AcrIIA4
(Plasmid
#136662)
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PurposeExpresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVAX_CBI
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAcrIIA4
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SpeciesH. sapiens (human), Synthetic
- Promoter CMV
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Tag
/ Fusion Protein
- No tag, No NLS
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAT GGT TGG GAT AAG GCT GG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVAX_CBI_AcrIIA4 was a gift from Yannick Doyon (Addgene plasmid # 136662 ; http://n2t.net/addgene:136662 ; RRID:Addgene_136662) -
For your References section:
Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins. Hynes AP, Rousseau GM, Agudelo D, Goulet A, Amigues B, Loehr J, Romero DA, Fremaux C, Horvath P, Doyon Y, Cambillau C, Moineau S. Nat Commun. 2018 Jul 25;9(1):2919. doi: 10.1038/s41467-018-05092-w. 10.1038/s41467-018-05092-w PubMed 30046034
