Tet1-dCas9
(Plasmid #136650)

• Purpose: Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
• Depositing Lab: David Segal
• Publication: Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s41416-022-01812-8. doi: 10.1038/s41416-022-01812-8.

Backbone

• Vector backbone: pcDNA3.3-TOPO
• Backbone manufacturer: ThermoFisher
• Backbone size w/o insert (bp): 5407
• Total vector size (bp): 11944
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tet1
• Alt name: CXXC6
• Species: M. musculus (mouse)
• Insert Size (bp): 2019
• GenBank ID:
• Entrez Gene: Tet1 (a.k.a. 2510010B09Rik, Cxxc6, D10Ertd17e, LCX, mKIAA1676)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLag-NLS-Tet1-dCas9-NLS (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: polyA TK-R

Resource Information

• Supplemental Documents:
  • TET1-dCas9.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry