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pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
(Plasmid #136646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4-TO-mychis
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SH3BP1R312A (RNAi resistant)
  • Species
    H. sapiens (human)
  • Mutation
    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC and R314 to A
  • Entrez Gene
    SH3BP1 (a.k.a. ARHGAP43)
  • Tag / Fusion Protein
    • HA (MQDLPGNDNSTAG) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Deficient in GAP activity due to substitution of R314

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-TO-HA-SH3BP1 R312A (RNAi-res) was a gift from Maria Balda & Karl Matter (Addgene plasmid # 136646 ; http://n2t.net/addgene:136646 ; RRID:Addgene_136646)

  • For your References section:

    Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex. Elbediwy A, Zihni C, Terry SJ, Clark P, Matter K, Balda MS. J Cell Biol. 2012 Aug 20;198(4):677-93. doi: 10.1083/jcb.201202094. Epub 2012 Aug 13. 10.1083/jcb.201202094 PubMed 22891260
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