pCB6-OccludinCT3
(Plasmid
#136639)
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PurposeExpression of OccludinCT3 in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCB6
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameOccludinCT3
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SpeciesG. gallus (chicken)
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MutationDeletion of C-terminal cytoplasid domain
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Entrez GeneOCLN
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCB6-OccludinCT3 was a gift from Maria Balda & Karl Matter (Addgene plasmid # 136639 ; http://n2t.net/addgene:136639 ; RRID:Addgene_136639) -
For your References section:
Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. Balda MS, Whitney JA, Flores C, Gonzalez S, Cereijido M, Matter K. J Cell Biol. 1996 Aug;134(4):1031-49. doi: 10.1083/jcb.134.4.1031. 10.1083/jcb.134.4.1031 PubMed 8769425
