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Addgene

pCB6-HAOccludin
(Plasmid #136638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136638 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCB6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HAOccludin
  • Species
    G. gallus (chicken)
  • Entrez Gene
    OCLN
  • Tag / Fusion Protein
    • HA (MQDLPGNDNSTAG) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB6-HAOccludin was a gift from Maria Balda & Karl Matter (Addgene plasmid # 136638 ; http://n2t.net/addgene:136638 ; RRID:Addgene_136638)

  • For your References section:

    Functional dissociation of paracellular permeability and transepithelial electrical resistance and disruption of the apical-basolateral intramembrane diffusion barrier by expression of a mutant tight junction membrane protein. Balda MS, Whitney JA, Flores C, Gonzalez S, Cereijido M, Matter K. J Cell Biol. 1996 Aug;134(4):1031-49. doi: 10.1083/jcb.134.4.1031. 10.1083/jcb.134.4.1031 PubMed 8769425
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