pET51b(+)_EGFP_NbALFA
(Plasmid #136626)

• Purpose: Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion
• Depositing Lab: Kai Johnsson
• Publication: Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7.

Backbone

• Vector backbone: pET51-b(+)
• Backbone manufacturer: Novagen
• Total vector size (bp): 6277
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EGFP-NbALFA
• Species: Synthetic
• Insert Size (bp): 1215
• Promoter: T7
• Tags / Fusion Proteins:
  • Strep (N terminal on insert)
  • Hisx10 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: RP

Resource Information

• Supplemental Documents:
  • pET51b(_)_EGFP_NbALFA.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry