pCDNA3.0_nucLAMA-G97 IRES EGFP
(Plasmid
#136620)
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PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3.0
- Total vector size (bp) 8272
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
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SpeciesSynthetic
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Insert Size (bp)1512
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV_Fwd
- 3′ sequencing primer BGH_Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3.0_nucLAMA-G97 IRES EGFP was a gift from Kai Johnsson (Addgene plasmid # 136620 ; http://n2t.net/addgene:136620 ; RRID:Addgene_136620) -
For your References section:
Chemogenetic Control of Nanobodies. Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7. 10.1038/s41592-020-0746-7 PubMed 32066961
