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pET-11a (D3 - F3Y)
(Plasmid #136604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5677
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DANCER-3 mutant (F3Y reversion)
  • Alt name
    Streptococcal protein G, domain B1 mutant
  • Species
    Streptococcal bacteria
  • Insert Size (bp)
    168
  • Mutation
    Mutation from GB1 WT: L7I-A34F-V39L-V54I
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x-Histag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer NdeI restriction site
  • 3′ sequencing primer BamHI restriction site
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-11a (D3 - F3Y) was a gift from Roberto Chica (Addgene plasmid # 136604 ; http://n2t.net/addgene:136604 ; RRID:Addgene_136604)

  • For your References section:

    Origin of conformational dynamics in a globular protein. Damry AM, Mayer MM, Broom A, Goto NK, Chica RA. Commun Biol. 2019 Nov 26;2:433. doi: 10.1038/s42003-019-0681-2. eCollection 2019. 10.1038/s42003-019-0681-2 PubMed 31799435
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