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pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140
(Plasmid #136601)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-EF1a-IRES-Blast
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-mSOG-FRB-P2A-T2A-FKBP-C-mSOG
  • Promoter EF1A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1A Foward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 was a gift from John Ngo (Addgene plasmid # 136601 ; http://n2t.net/addgene:136601 ; RRID:Addgene_136601)

  • For your References section:

    Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Boassa D, Lemieux SP, Lev-Ram V, Hu J, Xiong Q, Phan S, Mackey M, Ramachandra R, Peace RE, Adams SR, Ellisman MH, Ngo JT. Cell Chem Biol. 2019 Oct 17;26(10):1407-1416.e5. doi: 10.1016/j.chembiol.2019.07.007. Epub 2019 Aug 1. 10.1016/j.chembiol.2019.07.007 PubMed 31378710
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