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Addgene

pLNCX chick src Y527F
(Plasmid #13660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLNCX
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    src Y527F
  • Alt name
    src
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1900
  • Mutation
    Y527F. Constitutive active mutant.
  • Entrez Gene
    SRC (a.k.a. PP60C-SCR, SDR, c-src)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer LNCX primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNCX chick src Y527F was a gift from Joan Brugge (Addgene plasmid # 13660 ; http://n2t.net/addgene:13660 ; RRID:Addgene_13660)
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