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pcDNA3-N-mSOG-bFos-IRES-mCherry
(Plasmid #136599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 7466
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-mSOG-bFos
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-N-mSOG-bFos-IRES-mCherry was a gift from John Ngo (Addgene plasmid # 136599 ; http://n2t.net/addgene:136599 ; RRID:Addgene_136599)

  • For your References section:

    Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy. Boassa D, Lemieux SP, Lev-Ram V, Hu J, Xiong Q, Phan S, Mackey M, Ramachandra R, Peace RE, Adams SR, Ellisman MH, Ngo JT. Cell Chem Biol. 2019 Oct 17;26(10):1407-1416.e5. doi: 10.1016/j.chembiol.2019.07.007. Epub 2019 Aug 1. 10.1016/j.chembiol.2019.07.007 PubMed 31378710
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