DmTmem63
(Plasmid #136598)

• Purpose: Expresses the mechanosensitive channel Drosophila melanogaster Tmem63 in mammalian cells
• Depositing Lab: Ardem Patapoutian
• Publication: Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10.7554/eLife.41844.

Backbone

• Vector backbone: pIRES2-mCherry
• Backbone size w/o insert (bp): 5286
• Total vector size (bp): 7568
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DmTMEM63
• Species: D. melanogaster (fly)
• Insert Size (bp): 7569
• GenBank ID: Dmel_CG11210
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES Reverse

Resource Information

• Supplemental Documents:
  • dTmem63a-codon_opti-pIres2-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: DmTmem63 was synthesized and human codon-optimized by Genewiz.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry