OSCA4.1
(Plasmid #136597)

• Purpose: Expresses the mechanosensitive channel OSCA4.1 in mammalian cells
• Depositing Lab: Ardem Patapoutian
• Publication: Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10.7554/eLife.41844.

Backbone

• Vector backbone: pIRES2-mCherry
• Backbone size w/o insert (bp): 5262
• Total vector size (bp): 7727
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OSCA4.1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2466
• GenBank ID: NM_119753.3
• Entrez Gene: AT4G35870 (a.k.a. F4B14.140, F4B14_140)
• Promoter: CMV
• Tag / Fusion Protein:
  • pIRES2-mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES Reverse

Resource Information

• Supplemental Documents:
  • OSCA4.1-codon_opti-pIRES2-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: Sequence was downloaded from TAIR [The arabidopsis information resource] and human codon-optimized and synthesized by Genewiz.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry