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Addgene

OSCA1.1
(Plasmid #136592)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136592 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2-mCherry
  • Backbone size w/o insert (bp) 5286
  • Total vector size (bp) 7604
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OSCA1.1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    2319
  • GenBank ID
    NM_178966.1
  • Entrez Gene
    AT4G04340 (a.k.a. T19B17.6, T19B17_6)
  • Promoter CMV
  • Tag / Fusion Protein
    • pIRES2-mCherry (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Sequence was downloaded from TAIR [The arabidopsis information resource] and human codon-optimized and synthesized by Genewiz.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OSCA1.1 was a gift from Ardem Patapoutian (Addgene plasmid # 136592 ; http://n2t.net/addgene:136592 ; RRID:Addgene_136592)
  • For your References section:

    OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels. Murthy SE, Dubin AE, Whitwam T, Jojoa-Cruz S, Cahalan SM, Mousavi SAR, Ward AB, Patapoutian A. Elife. 2018 Nov 1;7. pii: 41844. doi: 10.7554/eLife.41844. 10.7554/eLife.41844 PubMed 30382938