OSCA1.1
(Plasmid
#136592)
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PurposeExpresses the mechanosensitive channel OSCA1.1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136592 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIRES2-mCherry
- Backbone size w/o insert (bp) 5286
- Total vector size (bp) 7604
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSCA1.1
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)2319
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GenBank IDNM_178966.1
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Entrez GeneAT4G04340 (a.k.a. T19B17.6, T19B17_6)
- Promoter CMV
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Tag
/ Fusion Protein
- pIRES2-mCherry (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer IRES Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySequence was downloaded from TAIR [The arabidopsis information resource] and human codon-optimized and synthesized by Genewiz.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OSCA1.1 was a gift from Ardem Patapoutian (Addgene plasmid # 136592 ; http://n2t.net/addgene:136592 ; RRID:Addgene_136592) -
For your References section:
OSCA/TMEM63 are an Evolutionarily Conserved Family of Mechanically Activated Ion Channels. Murthy SE, Dubin AE, Whitwam T, Jojoa-Cruz S, Cahalan SM, Mousavi SAR, Ward AB, Patapoutian A. Elife. 2018 Nov 1;7. pii: 41844. doi: 10.7554/eLife.41844. 10.7554/eLife.41844 PubMed 30382938