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pBABE_hygro_mRFP1_NRF2(C506S, L4A)
(Plasmid #136580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone manufacturer
    Addgene #1765
  • Backbone size w/o insert (bp) 5219
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NRF2 (C506S, Leuzip mut)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2530
  • Mutation
    The C506S mutation (C513S in the actual sequence of this plasmid) and leucine zipper (ZIP) mutation (L553A_L557A_L560A_L562A) were performed by site-directed mutagenesis of the pBabe mRFP1-Nrf2 hygro plasmid.
  • GenBank ID
    NM_006164
  • Entrez Gene
    NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
  • Tag / Fusion Protein
    • N-mRFP1-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE_hygro_mRFP1_NRF2(C506S, L4A) was a gift from Kevin Janes (Addgene plasmid # 136580 ; http://n2t.net/addgene:136580 ; RRID:Addgene_136580)

  • For your References section:

    Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins KA, Wang CC, Janes KA. Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200. 10.1126/scisignal.aba4200 PubMed 32291314
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