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Addgene

pmiRFP680-tubulin
(Plasmid #136575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136575 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miRFP680-tubulin
  • Species
    H. sapiens (human), Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • miRFP680 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiRFP680-tubulin was a gift from Vladislav Verkhusha (Addgene plasmid # 136575 ; http://n2t.net/addgene:136575 ; RRID:Addgene_136575)

  • For your References section:

    A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti F, Shemetov AA, Testa I, Verkhusha VV. Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6. 10.1038/s41467-019-13897-6 PubMed 31932632
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