pCMV5 mouse src K295R Y527F
(Plasmid #13657)

• Depositing Labs: Joan Brugge, Peter Howley
• Publication: src plasmids (unpublished)

Backbone

• Vector backbone: pCMV5 *
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: src K295R Y527F
• Alt name: c-src
• Alt name: src
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Mutation: K295R Y527F. Dominant negative mutant.
• Entrez Gene: Src (a.k.a. pp60c-src)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

* The depositing lab is not completely sure of the backbone for this plasmid; Addgene's sequencing results indicate that it may instead be pcDNA3. The plasmid should still function correctly, but users should consult Addgene's sequencing results and exercise caution if subcloning is necessary.