pvimentin-miRFP713
(Plasmid #136569)

• Purpose: Vimentin labeling with near-infrared fluorescent protein miRFP713
• Depositing Lab: Vladislav Verkhusha
• Publication: Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6.

Backbone

• Vector backbone: pN1
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: vimentin-miRFP713
• Species: H. sapiens (human), Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • miRFP713 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • pvimentin-miRFP713.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry