pCL52.2
(Plasmid
#13656)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
- Backbone size (bp) 7912
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Vector typeE. coli-S. arueus shuttle vector
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Selectable markerstetracycline in S. aureus at 30C
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCL52.2 was a gift from Chia Lee (Addgene plasmid # 13656 ; http://n2t.net/addgene:13656 ; RRID:Addgene_13656) -
For your References section:
Molecular characterization and transcriptional analysis of type 8 capsule genes in Staphylococcus aureus. Sau S, Sun J, Lee CY. J Bacteriol. 1997 Mar . 179(5):1614-21. PubMed 9045821
