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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)
(Plasmid #136546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136546 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • ANXA2 (23-339) (C terminal on insert)
    • His-tag (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

More data in this paper:

Structure determination of the transactivation domain of p53 in complex with S100A4 using annexin A2 as a crystallization chaperone

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339) was a gift from Laszlo Nyitray (Addgene plasmid # 136546 ; http://n2t.net/addgene:136546 ; RRID:Addgene_136546)

  • For your References section:

    Structure Determination of the Transactivation Domain of p53 in Complex with S100A4 Using Annexin A2 as a Crystallization Chaperone. Ecsedi P, Gogl G, Hof H, Kiss B, Harmat V, Nyitray L. Structure. 2020 Aug 4;28(8):943-953.e4. doi: 10.1016/j.str.2020.05.001. Epub 2020 May 21. 10.1016/j.str.2020.05.001 PubMed 32442400
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