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Addgene

pSLIK Zeo p53DD
(Plasmid #136534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK-Zeo
  • Backbone manufacturer
    Addgene #25736
  • Backbone size w/o insert (bp) 12944
  • Vector type
    Mammalian Expression, Lentiviral ; destinatioin vector for gateway cloning
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    **Grow plasmid in amp+ 25 ug/ml zeo in low-salt LB at 30degC.**
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53DD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    309
  • Mutation
    This construct was created from the pBabe p53DD (addgene #9058).
  • GenBank ID
    NM_011640.3
  • Entrez Gene
    Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK Zeo p53DD was a gift from Kevin Janes (Addgene plasmid # 136534 ; http://n2t.net/addgene:136534 ; RRID:Addgene_136534)

  • For your References section:

    Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins KA, Wang CC, Janes KA. Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200. 10.1126/scisignal.aba4200 PubMed 32291314
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